DNA
IT1

Part:BBa_K5170004:Design

Designed by: Teng, I-Ting., Li, X., Yadikar, H. A., Yang, Z., Li, L., Lyu, Y., Pan, X., Wang, K. K., & Tan, W.   Group: iGEM24_IISc-Bengaluru   (2024-10-01)


DNA Aptamer against 231pTau


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since only the forward single strand is the binder, it is vital to either denature and separate the strands if propagated by rounds of PCR. It can be done either by Biotin labelling the forward primer, denaturing the PCR product with NaOH and separating by passing through Streptavidin immobilised on Sepharose or Agarose; or by simply incubating the PCR product with lambda-exonuclease, if Biotin labelling is not used.


Source

It was selected by SELEX; randomisation of a library followed by negative and positive binding selection rounds.

References

Teng, I-Ting., Li, X., Yadikar, H. A., Yang, Z., Li, L., Lyu, Y., Pan, X., Wang, K. K., & Tan, W. (2018). Identification and Characterization of DNA Aptamers Specific for Phosphorylation Epitopes of Tau Protein. Journal of the American Chemical Society, 140(43), 14314–14323. https://doi.org/10.1021/jacs.8b08645.